Scientist – Assay Development

Scientist – Assay Development

Pittsburgh, PA

Job Title: Scientist – Assay Development
FLSA Status: Full-Time/Exempt
Department: Discovery
Date: April 22, 2022
Manager: SVP, R&D
Location: Pittsburgh, PA
Job Code: BIO-22-009

BlueSphere Bio was founded to deploy its novel TCXpress™ technology to unlock the potential of personalized T cell therapy for the treatment of cancer by harnessing the unique specificity of each patient’s T cells for the molecular fingerprint of each patient’s cancer.

Our trailblazing, proprietary TCXpress platform is an elegant, rapid approach that exploits the patient’s immune defenses to attack their cancer specifically and is designed to leave normal tissues untouched, thereby reducing harmful side effects.

The TCXpress™ platform and its implementation will allow us to deliver rapid, personalized T cell treatments for various cancer indications.

We are seeking an exceptionally talented and highly motivated Ph.D. Scientist with immunology and/or oncology background to join the Assay Development Group at BlueSphere Bio. The primary role of this position is to support the continued development of a high-throughput, sensitive, and automation-compatible T cell receptor screening platform. Preference will be given to individuals with extensive knowledge in high content imaging/fluorescent microscopy and/or a background in robotic assay automation. The ideal candidate will have a proven track record of advancing projects in the form of publications and/or patents and possess a scientifically creative (i.e., problem-solving) mind. Excellent communication skills and self-organization are required, as the candidate is expected to operate largely independently in a fast-paced, results-driven work environment within the Assay Development team.

Duties and Responsibilities
• Characterize, implement, and contribute to the continued development of a high-throughput fluorescence-microscopy based screening assay
• Independent design, execution, and analysis of experiments
• Routine interpretation and presentation of scientific data
• Utilize broad technical expertise in T cell immunology and molecular biology to propose novel mechanisms to improve the signal:noise ratio in high throughput screens
• Collaborate with other BSB scientists for the advancement of new technologies and participate in patent writing
• Clearly communicate, present, and defend critical activities for program progression
• Excellent time management skills and ability to meet aggressive deliverable timelines
• Other duties as assigned

Qualifications and Work Experience
• Ph.D. in Immunology, Oncology, Molecular Biology, or related field with 1-3 years of post-graduate experience
• In depth knowledge of T cell receptor signal transduction and/or characteristics of T cell activation
• Demonstrated experience with immunofluorescence microscopy
• Flow cytometry expertise (panel design, implementation, and troubleshooting)
• Experience with high throughput cellular assays (in the form of development and/or execution)
• Excellent verbal and written skills
• Scientific ingenuity and creativity
• Preferred: Familiarity with advanced molecular biology techniques
• Preferred: Familiarity with lentivirus design, production, and transduction
• Preferred: Cellular engineering experience (Cas9 editing, targeted knock-in)

Physical and Mental Requirements
• You will be expected to occasionally (1% to 20% of the time):
– Climb stairs, ramps, ladders. Etc.
– Stoop/crouch by bending your legs or bending down/forward at the waist
– Reach by extending the hands/arms
– Stand on your feet
– Lift objects from one level to another (30 lbs. or less)
– Carry objects (30 lbs. or less)
– Travel (negligible)
• You will be expected to frequently (21% to 50% of the time):
– Walk around the facility on foot
• You will be expected to constantly (at least 51% of the time):
– Sit in a normal seated position

BlueSphere Bio is an Equal Opportunity Employer (EOE) and offers employment opportunities to all qualified persons regardless of race, color, religion, sex, age, national origin or ancestry, physical or mental disability (except where physical or mental abilities are a bona fide occupational requirement), veteran status, marital status, familial status, sexual orientation, HIV-positive status, possession of the sickle cell trait, genetic characteristics, political views, and any other basis protected by federal, state or local laws.

Please send a cover letter to apply for this position (including job code) and CV/Resume. Internal candidates interested in this opportunity should submit their CV/Resume within 2-weeks of the posting date above and explain why you are interested in this position.