Research Associate – Product Development

Research Associate – Product Development

Pittsburgh, PA

Job Title: Research Associate – Discovery
FLSA Status: Full-Time/Exempt
Department: Clinical Development
Date: February 4, 2022
Manager: SVP, R&D
Location: Pittsburgh, PA
Job Code: BIO-22-002

BlueSphere Bio was founded to deploy its novel TCXpress™ technology to unlock the potential of personalized T cell therapy for the treatment of cancer by harnessing the unique specificity of each patient’s T cells for the molecular fingerprint of each patient’s cancer.

Our trailblazing, proprietary TCXpress platform is an elegant, rapid approach that exploits the patient’s immune defenses to attack their cancer specifically and is designed to leave normal tissues untouched, thereby reducing harmful side effects.

The TCXpress™ platform and its implementation will allow us to deliver rapid, personalized T cell treatments for various cancer indications.

The Research Associate will work on process development for our high throughput TCR screening platform, utilizing a range of molecular biology and cell culture techniques. In addition, the incumbent will work on process development for our High Throughput neoantigen screening platform (NeoXpress), using a range of Molecular Biology and Cell Culture techniques. They would assist in the process implementation of the NeoXpress platform and on assay development, miniaturization, automation, and implementation of high throughput functional screening assays. The Research Associate will join a team performing diverse functions, assisting in TCR leads’ preclinical advancement and product characterization in early clinical development.

Duties and Responsibilities
• Organize and support the NeoXpress platform operations.
• Using standard Molecular Biology techniques, prepare, and QC reagents used for standard operating procedures, including DNA plasmid purification, master mixes for PCR reactions, real-time PCR, and cloning reagents.
• Responsible for scheduling, organizing, and performing campaigns in collaboration with the NeoXpress screening assay group.
• Using cloning and sterile culture techniques, maintain and establish new cell lines for the functional characterization of Neoantigen and T cell receptor pairs.
• Participate in developing and standardizing cell-based assays in high throughput format using liquid handlers and automated culturing instruments.
• Characterize cells using multi-parameter Flow Cytometry and Data Analysis.
• Maintain accurate sample and laboratory records, including human tissue biobanking. Using LIMS, record and analyze data of processed samples.
• Organize and conduct assigned studies independently, following and adhering to current experimental protocols.
• Present regularly to management and team meetings.
• Work closely with our cross-functional research and discovery teams.
• Other duties as assigned

Qualifications and Work Experience
• Bachelor’s or master’s degree in Immunology, Oncology, Cell Biology, Molecular biology or equivalent experience required, with >4 years of working experience preferred
• Experience in Biotech/ Pharmaceutical industry is preferred.
• Demonstrated work in Molecular Biology, including cloning, and gene manipulation, including bioinformatics analysis.
• Hands-on experience with multi-parameter Flow Cytometry and data analysis is preferred but not required.
• Experience in basic cell culturing techniques.
• Experience with automation and robotic instrumentation is an advantage.
• Previous experience in Laboratory Information Management Systems preferred.
• Proficiency with analytical software, e.g., PRISM, FlowJo, Excel
• Efficiency and organization, with the ability to adhere to timelines and milestones
• Excellent communications and presentation skills are highly important.

BlueSphere Bio is an Equal Opportunity Employer (EOE) and offers employment opportunities to all qualified persons regardless of race, color, religion, sex, age, national origin or ancestry, physical or mental disability (except where physical or mental abilities are a bona fide occupational requirement), veteran status, marital status, familial status, sexual orientation, HIV-positive status, possession of the sickle cell trait, genetic characteristics, political views, and any other basis protected by federal, state or local laws.

Please send a cover letter to apply for this position (including job code) and CV/Resume. Internal candidates interested in this opportunity should submit their CV/Resume within 2-weeks of the posting date above and explain why you are interested in this position.