Research Associate – Discovery

Research Associate – Discovery

Pittsburgh, PA

Job Title: Research Associate – Discovery
FLSA Status: Full-Time/Exempt
Department: Clinical Development
Date: August 25, 2021
Manager: SVP, R&D
Location: Pittsburgh, PA
Job Code: BIO-21-019

BlueSphere Bio was founded to deploy its novel TCXpress™ technology to unlock the potential of personalized T cell therapy for the treatment of cancer by harnessing the unique specificity of each patient’s T cells for the molecular fingerprint of each patient’s cancer.

Our trailblazing, proprietary TCXpress platform is an elegant, rapid approach that exploits the patient’s immune defenses to attack their cancer specifically and is designed to leave normal tissues untouched, thereby reducing harmful side effects.

The TCXpress™ platform and its implementation will allow us to deliver rapid, personalized T cell treatments for various cancer indications.

The Research Associate will work on process development for our high throughput TCR screening platform, utilizing a range of molecular biology and cell culture techniques. In addition, the associate will work on assay development, miniaturization, automation, and implementation of high throughput functional screening assays using BSB’s TCXpress and NeoXpress platforms. The Research Associate will join a team performing diverse functions, assisting in TCR leads’ preclinical advancement and product characterization in early clinical development. The position reports to the SVP, Research, and Development and is located in Pittsburgh, Pennsylvania.

Duties and Responsibilities
• TCR screening operations by assisting in the development and implementation of novel strategies to identify antigen-specific TCRs.
• PCR, cloning, and colony screening.
• Bacterial transformation, propagation, and purification of plasmid DNA
• Lentivirus production, purification, and banking
• Development and implementation of co-culture assays to identify novel TCRs
• Sterile culture techniques and cell sorting (including at single-cell level) to maintain and establish new cell lines for the functional characterization of TCRs
• Participate in developing and standardizing cellular-based assays in high throughput format using liquid handlers and automated culturing instruments.
• Characterize cells using multiparameter flow cytometry and subsequent data analysis.
• Maintain accurate sample and laboratory records, including human tissue and cell line biobanking.
• Continuous management of multiple, diverse cell lines
• Organize and conduct assigned studies independently. Importantly, the incumbent must be cable of troubleshooting various aspects of assay implementation.
• Present regularly to management and team meetings.
• Other duties as assigned

Qualifications and Work Experience
• Bachelor’s or Master’s degree in Immunology, Oncology, Cell Biology, Molecular biology, or equivalent experience required, with >4 years of working experience preferred.
• Demonstrated work in molecular biology, including cloning and gene manipulation.
• Hands-on experience with flow cytometry and data analysis is preferred.
• Proficiency with analytical software, e.g., PRISM, FlowJo, Excel
• Efficiency and organization, with the ability to adhere to timelines and milestones
• Excellent communication and presentation skills are essential.
• Demonstrated ability to independently identify issues and troubleshoot as necessary

BlueSphere Bio is an Equal Opportunity Employer (EOE) and offers employment opportunities to all qualified persons regardless of race, color, religion, sex, age, national origin or ancestry, physical or mental disability (except where physical or mental abilities are a bona fide occupational requirement), veteran status, marital status, familial status, sexual orientation, HIV-positive status, possession of the sickle cell trait, genetic characteristics, political views, and any other basis protected by federal, state or local laws.

Please send a cover letter to apply for this position (including job code) and CV/Resume. Internal candidates interested in this opportunity should submit their CV/Resume within 2-weeks of the posting date above and explain why you are interested in this position.