Laboratory Technician

Laboratory Technician

Pittsburgh, PA

Job Title: Laboratory Technician
FLSA Status: Full-Time/Exempt
Department: Operations
Date: October 4, 2021
Manager: SVP R&D
Location: Pittsburgh, PA
Job Code: BIO-21-021

BlueSphere Bio was founded to deploy its novel TCXpress™ technology to unlock the potential of personalized T cell therapy for the treatment of cancer by harnessing the unique specificity of each patient’s T cells for the molecular fingerprint of each patient’s cancer.

Our trailblazing, proprietary TCXpress™ platform is an elegant, rapid approach that exploits the patient’s immune defenses to attack their cancer specifically and is designed to leave normal tissues untouched, thereby reducing harmful side effects.

The TCXpress™ platform and its implementation will deliver rapid, personalized T cell treatments for various cancer indications.

This is an exciting opportunity for a Laboratory Manager to join our group to assist in developing novel therapeutics in the field of TCR-based Adoptive T cell therapies (ACTs).

The Lab Technician will develop a series of in vitro and ex vivo functional assays to characterize neoantigen-specific T cell receptors, utilizing a range of Molecular Biology and Cell Culture techniques. The Lab Technician would assist in-process implementation of the TXCpress platform and assay development, miniaturization, and implementation of high throughput functional screening assays. The Lab Technician will join a team performing diverse functions, assisting in the preclinical advancement of TCR leads and product characterization in early clinical development. The Laboratory Technician position reports to the Senior Vice President of Research and Development and is located in Pittsburgh, Pennsylvania.

Duties and Responsibilities

  • Organize and support the TCXpress platform operations.
  • Using standard Molecular Biology techniques, prepare, and QC reagents used for standard operating procedures, including DNA plasmid purification, master mixes for PCR reactions, real-time PCR, and cloning reagents.
  • Responsible for scheduling, organizing, and performing campaigns in collaboration with the TCXpress screening assay group.
  • Using cloning and sterile culture techniques, maintain and establish new cell lines for the functional characterization of T cell receptors.
  • Participate in developing and standardizing cell-based assays in high throughput format using liquid handlers and automated culturing instruments.
  • Characterize cells using multi-parameter Flow Cytometry and Data Analysis.
  • Maintain accurate sample and laboratory records, including human tissue biobanking. Using LIMS, record and analyze data of processed samples.
  • Organize and conduct assigned studies independently, following and adhering to current experimental protocols.
  • Present regularly to management and team meetings.
  • Work closely with our clinical team to advance and validate lead TCR candidates.
  • Other duties as assigned

Qualifications and Work Experience

  • Bachelor’s or Master’s degree in Immunology, Oncology, or Cell Biology or equivalent experience required, with >2 years of working experience preferred.
  • Experience in Biotech/ Pharmaceutical industry is preferred.
  • Demonstrated work in Molecular Biology including RT-PCR, qPCR, cloning, and gene manipulation, including bioinformatic analysis.
  • Hands-on experience with multi-parameter Flow Cytometry and data analysis is preferred but not required.
  • Experience in basic cell culturing techniques.
  • Experience with automation and robotic instrumentation is an advantage.
  • Previous experience in Laboratory Information Management Systems preferred.
  • Proficiency with analytical software, e.g., PRISM, FlowJo, Excel
  • Efficiency and organization, with the ability to adhere to timelines and milestones
  • Excellent communications and presentation skills are essential.

Physical and Mental Requirements

  • You will be expected to occasionally (1% to 20% of the time):
    o Climb stairs, ramps, ladders. Etc.
    o Stoop/crouch by bending your legs or bending down/forward at the waist o Reach by extending the hands/arms
    o Travel (<5%)
  • You will be expected to frequently (21% to 50% of the time): o Lift objects from one level to another (30 lbs. or less)
    o Carry objects (30 lbs. or less)
    o Walk around the facility on foot
    o Stand on your feet
  • You will be expected to constantly (at least 51% of the time):
    o Sit in a normal seated position

BlueSphere Bio is an Equal Opportunity Employer (EOE) and offers employment opportunities to all qualified persons regardless of race, color, religion, sex, age, national origin or ancestry, physical or mental disability (except where physical or mental abilities are a bona fide occupational requirement), veteran status, marital status, familial status, sexual orientation, HIV-positive status, possession of the sickle cell trait, genetic characteristics, political views, and any other basis protected by federal, state or local laws.

Please send a cover letter to apply for this position (including job code) and a CV/Resume. Internal candidates interested in this opportunity should submit their CV/Resume within 2-weeks of the posting date above and explain why you are interested in this position.